cfx384tm touch real time pcr detection system (Bio-Rad)
Bio-Rad is a verified supplier 
Bio-Rad manufactures this product 
96
Structured Review
Bio-Rad
cfx384tm touch real time pcr detection system
Cfx384tm Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx384tm+touch+real+time+pcr+detection+system/Hard-Shell+384-Well+PCR+Plates/pm41894916-87-20-26
Average 96 stars, based on 324 article reviews
Cfx384tm Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx384tm+touch+real+time+pcr+detection+system/Hard-Shell+384-Well+PCR+Plates/pm41894916-87-20-26
Average 96 stars, based on 324 article reviews
cfx384tm touch real time pcr detection system - by Bioz Stars,
2026-09
96/100 stars
Images
Related Articles
Polymerase Chain Reaction:Article Title: Sex-dimorphic growth hormone-releasing hormone (Ghrh) receptor regulation of ventromedial hypothalamic nucleus Ghrh neuron estrogen receptor variant gene expression Article Snippet: RT-qPCR analysis: IDTE (prod. code 11-05-01-05; 1× TE solution; Integrated DNA Technologies, Inc., Coralville, IA)-diluted pre-amplified cDNA samples (2 μL) were combined with Bio-Rad primers (ESR1 [0.5 μL; prod. code qRnoCID0009588], ESR2 [0.5 μL; prod. code qRnoCID0008785], GPER [0.5 μL; prod. code qRnoCED0007818], aromatase/CYP19A1 [0.5 μL; prod. code. qRnoCID0007793], Ghrh [0.5 μL; prod. code qRnoCID0007723], and GAPDH [0.5 μL; prod. code qRnoCID0057018]) and iTaqTM Universal SYBR ® Green Supermix (5 μL; prod. code 1725121; Bio-Rad). .. PCR samples were added to individual wells of 384-well hard-shell PCR plates (prod. code HSP3805, Bio-Rad) for analysis in a Article Title: Sex-dimorphic VMN Ghrh neuron metabolic sensor and neurotransmitter marker transcriptional adaptation to recurrent insulin-induced hypoglycemia. Article Snippet: Ventromedial hypothalamic nucleus/dorsomedial division (VMNdm) metabolic-sensory growth hormonereleasing hormone (Ghrh) neurons operate within the brain circuitry that maintains systemic glucostasis.. Sexspecific counterregulatory hormone adaptation to recurrent insulin-induced hypoglycemia (RIIH) entails undiscovered CNS mechanisms.. Combinative single-cell immunocytochemistry/laser-microdissection/multiplex qPCR methods were employed here to determine if RIIH alters VMNdm Ghrh neuron neurotransmission according to sex. Article Title: Aging Effects on Absolute and Relative Estrogen Receptor Variant Gene Expression Levels in Male Versus Female Rat Ventromedial Hypothalamic Nucleus Growth Hormone-Releasing Hormone Neurons Article Snippet: RT-qPCR analysis: cDNA samples were diluted with IDTE (185 μL; 11-05-01-05; Integrated DNA Technologies, Inc., Coralville, IA, USA). .. PCR samples were prepared by combining 2.0 μL cDNA sample with target gene Bio-Rad primers [ESR1 (0.5 μL; qRnoCID0009588), ESR2 (0.5 μL; qRnoCID0008785), GPER (0.5 μL; qRnoCED0007818), CYP19A1 (0.5 μL; qRnoCID0007793), Ghrh (0.5 μL; qRnoCID0007723), and GAPDH (0.5 μL; qRnoCID0057018)] and 5.0 μL iTaqTMUniversal SYBR® Green Supermix (1725121; BioRad), then added to individual wells of hard-shell 384- well PCR plates (HSP3805, Bio-Rad) for analysis in a BioRad Article Title: VMN growth hormone-releasing hormone receptor regulation of counterregulatory transmission Article Snippet: RT-qPCR Analysis: PCR samples were prepared by combining Bio-Rad primers [Ghrh-R (0.5 μL; prod. no. qRnoCED0003825), Ghrh (0.5 μL; prod. no. qRnoCID0007723), SF-1/NR5A1 (0.5 μL; prod. no. qRnoCID0001458), GAD1/GAD67 (0.5 μL; prod. no. qRnoCID0004554), GAD2/GAD65 (0.5 μL; prod. no. qRnoCID0003485), GLS (0.5 μL; prod. no. qRnoCID0007756), nNOS/NOS1 (0.5 μL; prod. no. qRnoCED0009301), AMPKα1 (0.5 μL; prod. no. qRnoCID0001262), AMPKα2 (0.5 μL; prod. no. qRnoCID0006799), and GAPDH (0.5 μL; prod. no. qRnoCID0057018)], cDNA sample (2 μL), and iTaqTM Universal SYBR® Green Supermix (5 μL, prod. no. 1725121; Bio-Rad). .. PCR samples were added to individual wells of hard-shell 384-well PCR plates (prod. no. HSP3805, Bio-Rad) for analysis in a Real-time Polymerase Chain Reaction:Article Title: Sex-dimorphic growth hormone-releasing hormone (Ghrh) receptor regulation of ventromedial hypothalamic nucleus Ghrh neuron estrogen receptor variant gene expression Article Snippet: RT-qPCR analysis: IDTE (prod. code 11-05-01-05; 1× TE solution; Integrated DNA Technologies, Inc., Coralville, IA)-diluted pre-amplified cDNA samples (2 μL) were combined with Bio-Rad primers (ESR1 [0.5 μL; prod. code qRnoCID0009588], ESR2 [0.5 μL; prod. code qRnoCID0008785], GPER [0.5 μL; prod. code qRnoCED0007818], aromatase/CYP19A1 [0.5 μL; prod. code. qRnoCID0007793], Ghrh [0.5 μL; prod. code qRnoCID0007723], and GAPDH [0.5 μL; prod. code qRnoCID0057018]) and iTaqTM Universal SYBR ® Green Supermix (5 μL; prod. code 1725121; Bio-Rad). .. PCR samples were added to individual wells of 384-well hard-shell PCR plates (prod. code HSP3805, Bio-Rad) for analysis in a Article Title: Sex-dimorphic VMN Ghrh neuron metabolic sensor and neurotransmitter marker transcriptional adaptation to recurrent insulin-induced hypoglycemia. Article Snippet: Ventromedial hypothalamic nucleus/dorsomedial division (VMNdm) metabolic-sensory growth hormonereleasing hormone (Ghrh) neurons operate within the brain circuitry that maintains systemic glucostasis.. Sexspecific counterregulatory hormone adaptation to recurrent insulin-induced hypoglycemia (RIIH) entails undiscovered CNS mechanisms.. Combinative single-cell immunocytochemistry/laser-microdissection/multiplex qPCR methods were employed here to determine if RIIH alters VMNdm Ghrh neuron neurotransmission according to sex. Article Title: Hindbrain Lactoprivic Regulation of Hypothalamic Third Ventricular Alpha-2 Tanycyte Metabolic Sensor Gene Expression in Hypoglycemic Male Versus Female Rats. Article Snippet: AMPK 5’-AMP-activated protein kinase AMPKα1/PRKAA1 AMPK catalytic subunit alpha-1 isoform AMPKα2/PRKAA2 AMPK catalytic subunit alpha-2 isoform ARH Arcuate hypothalamic nucleus CV4 Caudal fourth ventricle GCK Glucokinase GJA1/connexin-1 Gap junction protein alpha-1 GLUT2 Glucose transporter-2 IIH Insulin-induced hypoglycemia INS Insulin IR Infundibular recess KCNJ11/KATP Kir 6.2 ATP-sensitive potassium channel Kir 6.2 subunit Abbreviations α2-tanycytes Alpha2 tanycytes 3V Third ventricle Adra1a/α1a-AR Alpha1a-adrenergic receptor Adrb1/β1-AR Beta1-adrenergic receptor Article Title: DDR2 alleviates retinal vaso-obliteration and pathological neovascularization by modulating microglia M1/M2 phenotypic polarization in a mouse model of proliferative retinopathy. Article Snippet: Around 1000 ng of total RNA was used for cDNA synthesis using HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, Nanjing, China). .. Quantitative PCR was carried out by Article Title: CLM296: a highly selective inhibitor targeting ALDH1A3-driven tumor growth and metastasis in breast cancer Article Snippet: .. For both the in vitro studies, and tumor analysis, RNA was extracted from cells or minced tumors sections with TRizol (ThermoFisher Scientific) and the Purelink RNA mini kit (ThermoFisher Scientific), and reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) per manufacturer’s instructions. cDNA diluted (1:10) with Ultrapure RNase DNase free water (ThermoFisher Scientific, Invitrogen) was used in qPCR reactions with universal SYBR Green Supermix (Bio-Rad) with gene-specific primers ( Supplemental Table S1 ) and the qPCR was performed using the Article Title: VMN growth hormone-releasing hormone receptor regulation of counterregulatory transmission Article Snippet: RT-qPCR Analysis: PCR samples were prepared by combining Bio-Rad primers [Ghrh-R (0.5 μL; prod. no. qRnoCED0003825), Ghrh (0.5 μL; prod. no. qRnoCID0007723), SF-1/NR5A1 (0.5 μL; prod. no. qRnoCID0001458), GAD1/GAD67 (0.5 μL; prod. no. qRnoCID0004554), GAD2/GAD65 (0.5 μL; prod. no. qRnoCID0003485), GLS (0.5 μL; prod. no. qRnoCID0007756), nNOS/NOS1 (0.5 μL; prod. no. qRnoCED0009301), AMPKα1 (0.5 μL; prod. no. qRnoCID0001262), AMPKα2 (0.5 μL; prod. no. qRnoCID0006799), and GAPDH (0.5 μL; prod. no. qRnoCID0057018)], cDNA sample (2 μL), and iTaqTM Universal SYBR® Green Supermix (5 μL, prod. no. 1725121; Bio-Rad). .. PCR samples were added to individual wells of hard-shell 384-well PCR plates (prod. no. HSP3805, Bio-Rad) for analysis in a Incubation:Article Title: Sex-dimorphic growth hormone-releasing hormone (Ghrh) receptor regulation of ventromedial hypothalamic nucleus Ghrh neuron estrogen receptor variant gene expression Article Snippet: RT-qPCR analysis: IDTE (prod. code 11-05-01-05; 1× TE solution; Integrated DNA Technologies, Inc., Coralville, IA)-diluted pre-amplified cDNA samples (2 μL) were combined with Bio-Rad primers (ESR1 [0.5 μL; prod. code qRnoCID0009588], ESR2 [0.5 μL; prod. code qRnoCID0008785], GPER [0.5 μL; prod. code qRnoCED0007818], aromatase/CYP19A1 [0.5 μL; prod. code. qRnoCID0007793], Ghrh [0.5 μL; prod. code qRnoCID0007723], and GAPDH [0.5 μL; prod. code qRnoCID0057018]) and iTaqTM Universal SYBR ® Green Supermix (5 μL; prod. code 1725121; Bio-Rad). .. PCR samples were added to individual wells of 384-well hard-shell PCR plates (prod. code HSP3805, Bio-Rad) for analysis in a Article Title: Sex-dimorphic VMN Ghrh neuron metabolic sensor and neurotransmitter marker transcriptional adaptation to recurrent insulin-induced hypoglycemia. Article Snippet: Ventromedial hypothalamic nucleus/dorsomedial division (VMNdm) metabolic-sensory growth hormonereleasing hormone (Ghrh) neurons operate within the brain circuitry that maintains systemic glucostasis.. Sexspecific counterregulatory hormone adaptation to recurrent insulin-induced hypoglycemia (RIIH) entails undiscovered CNS mechanisms.. Combinative single-cell immunocytochemistry/laser-microdissection/multiplex qPCR methods were employed here to determine if RIIH alters VMNdm Ghrh neuron neurotransmission according to sex. Article Title: Hindbrain Lactoprivic Regulation of Hypothalamic Third Ventricular Alpha-2 Tanycyte Metabolic Sensor Gene Expression in Hypoglycemic Male Versus Female Rats. Article Snippet: AMPK 5’-AMP-activated protein kinase AMPKα1/PRKAA1 AMPK catalytic subunit alpha-1 isoform AMPKα2/PRKAA2 AMPK catalytic subunit alpha-2 isoform ARH Arcuate hypothalamic nucleus CV4 Caudal fourth ventricle GCK Glucokinase GJA1/connexin-1 Gap junction protein alpha-1 GLUT2 Glucose transporter-2 IIH Insulin-induced hypoglycemia INS Insulin IR Infundibular recess KCNJ11/KATP Kir 6.2 ATP-sensitive potassium channel Kir 6.2 subunit Abbreviations α2-tanycytes Alpha2 tanycytes 3V Third ventricle Adra1a/α1a-AR Alpha1a-adrenergic receptor Adrb1/β1-AR Beta1-adrenergic receptor Article Title: VMN growth hormone-releasing hormone receptor regulation of counterregulatory transmission Article Snippet: RT-qPCR Analysis: PCR samples were prepared by combining Bio-Rad primers [Ghrh-R (0.5 μL; prod. no. qRnoCED0003825), Ghrh (0.5 μL; prod. no. qRnoCID0007723), SF-1/NR5A1 (0.5 μL; prod. no. qRnoCID0001458), GAD1/GAD67 (0.5 μL; prod. no. qRnoCID0004554), GAD2/GAD65 (0.5 μL; prod. no. qRnoCID0003485), GLS (0.5 μL; prod. no. qRnoCID0007756), nNOS/NOS1 (0.5 μL; prod. no. qRnoCED0009301), AMPKα1 (0.5 μL; prod. no. qRnoCID0001262), AMPKα2 (0.5 μL; prod. no. qRnoCID0006799), and GAPDH (0.5 μL; prod. no. qRnoCID0057018)], cDNA sample (2 μL), and iTaqTM Universal SYBR® Green Supermix (5 μL, prod. no. 1725121; Bio-Rad). .. PCR samples were added to individual wells of hard-shell 384-well PCR plates (prod. no. HSP3805, Bio-Rad) for analysis in a other:Article Title: Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programs Article Snippet: The oligonucleotide sequences for forward and reverse primers for E74-like ETS transcription factor 3 ( ELF3 ), retinoic acid receptor β ( RARβ ), dehydrogenase/reductase 3 ( DHRS3 ), human and mouse glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ), mucin 4 ( MUC4 ), tissue inhibitor of metalloproteinases-3 ( TIMP3 ), retinoic acid receptor responder 1 ( RARRES1 ), eyes absent homolog 2 ( EYA2 ), cytochrome P450 family 4 subfamily X member 1 ( CYP4X1 ), stimulated by retinoic acid gene 6 ( STRA6 ), adenosine diphosphate (ADP) ribosylation factor 1 ( ARF1 ), beta-2-microglobin ( β2M ), pumilio RNA binding family member 1 ( PUM1 ), ribosomal protein L29 ( RPL29 ), and TATA binding protein ( TBP ) ( ) were purchased from Life Technologies (Thermo Fisher Scientific). SYBR Green Assay:Article Title: DDR2 alleviates retinal vaso-obliteration and pathological neovascularization by modulating microglia M1/M2 phenotypic polarization in a mouse model of proliferative retinopathy. Article Snippet: Around 1000 ng of total RNA was used for cDNA synthesis using HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, Nanjing, China). .. Quantitative PCR was carried out by Article Title: CLM296: a highly selective inhibitor targeting ALDH1A3-driven tumor growth and metastasis in breast cancer Article Snippet: .. For both the in vitro studies, and tumor analysis, RNA was extracted from cells or minced tumors sections with TRizol (ThermoFisher Scientific) and the Purelink RNA mini kit (ThermoFisher Scientific), and reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) per manufacturer’s instructions. cDNA diluted (1:10) with Ultrapure RNase DNase free water (ThermoFisher Scientific, Invitrogen) was used in qPCR reactions with universal SYBR Green Supermix (Bio-Rad) with gene-specific primers ( Supplemental Table S1 ) and the qPCR was performed using the In Vitro:Article Title: CLM296: a highly selective inhibitor targeting ALDH1A3-driven tumor growth and metastasis in breast cancer Article Snippet: .. For both the in vitro studies, and tumor analysis, RNA was extracted from cells or minced tumors sections with TRizol (ThermoFisher Scientific) and the Purelink RNA mini kit (ThermoFisher Scientific), and reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) per manufacturer’s instructions. cDNA diluted (1:10) with Ultrapure RNase DNase free water (ThermoFisher Scientific, Invitrogen) was used in qPCR reactions with universal SYBR Green Supermix (Bio-Rad) with gene-specific primers ( Supplemental Table S1 ) and the qPCR was performed using the Reverse Transcription:Article Title: CLM296: a highly selective inhibitor targeting ALDH1A3-driven tumor growth and metastasis in breast cancer Article Snippet: .. For both the in vitro studies, and tumor analysis, RNA was extracted from cells or minced tumors sections with TRizol (ThermoFisher Scientific) and the Purelink RNA mini kit (ThermoFisher Scientific), and reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) per manufacturer’s instructions. cDNA diluted (1:10) with Ultrapure RNase DNase free water (ThermoFisher Scientific, Invitrogen) was used in qPCR reactions with universal SYBR Green Supermix (Bio-Rad) with gene-specific primers ( Supplemental Table S1 ) and the qPCR was performed using the cDNA Synthesis:Article Title: CLM296: a highly selective inhibitor targeting ALDH1A3-driven tumor growth and metastasis in breast cancer Article Snippet: .. For both the in vitro studies, and tumor analysis, RNA was extracted from cells or minced tumors sections with TRizol (ThermoFisher Scientific) and the Purelink RNA mini kit (ThermoFisher Scientific), and reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) per manufacturer’s instructions. cDNA diluted (1:10) with Ultrapure RNase DNase free water (ThermoFisher Scientific, Invitrogen) was used in qPCR reactions with universal SYBR Green Supermix (Bio-Rad) with gene-specific primers ( Supplemental Table S1 ) and the qPCR was performed using the |